Print | Text Size
AneuVysion Technology
AneuVysion technology (fluorescence in situ hybridization) overview
AneuVysion testing utilizes a technology called fluorescence in situ hybridization, or FISH. After initial processing of the amniotic fluid sample, five different DNA probes corresponding to chromosomes 13, 18, 21, X or Y, each labeled with one of three different colors of fluorescent tags, are hybridized to amniocytes bound to microscope slides containing two areas of identical amniocyte samples. Excess probe is washed from the slides, and then cells are visualized by laboratory technicians utilizing fluorescent microscopes. By counting the number of fluorescent signals of each color in a cell, it can be determined if the cell has a normal or abnormal number of copies of chromosomes 13, 18, 21, X and Y. Typically, 50 cells are analyzed by one or two technicians, and the results are usually reviewed by a Ph.D. laboratory director before AneuVysion results are reported to the referring doctor. AneuVysion is intended to be used as an aid in the diagnosis of abnormalities of chromosomes 13, 18, 21, X and/or Y in conjunction with other information currently used in prenatal diagnosis, consistent with professional standards of practice, for woman with presumed high risk pregnancies.
|
|
